What Plate to Use

To run just about any experiment on the microscope, you have to put cells in a dish, on a slide or in a microplate. Most of our experiments are run in a microplate, so I will concentrate on these style plates. Microtiter plate it turns out is a registered trademark of Cook Engineering Company, so I will use the generic term microplate (1). Depending on the experiment, there is a wide variety of microplates. The more common plates are 6, 12, 24, 48, 96 and 384 well plates. There are plates with yet higher numbers of wells: 1536, 3456 and 9600, these plates are very difficult to manually pipette into, designed for automation. There are different styles of plates as well, round and square as well as different volumes and different coatings. As with anything, it’s important to pick the best plate for the experiment and to be aware of some issues involved with plates. Since we do visible imaging and try to have multiple variations in each experiment, we shoot for smaller volume higher density plates. For example, if the experiment involves the effect of extract concentration on yeast cell growth, you will need a number of wells to look at the effect. Let’s say you want to look at 5 different concentrations of extract, then a negative control and a positive control, that’s 7 wells for one concentration of yeast. Next I may want to see if there is an affect with yeast concentration as well, so maybe … Continue reading